Oligo Pools

Twist currently does not offer modified oligos.

As of December 2025, Twist supports the use of the N degenerate base in Oligo Pools.

Customers may now incorporate N at specified positions within their oligo designs. At this time, other degenerate bases (R, Y, S, W, K, M, B, D, H, V) are not supported. If you wish to include a defined subset of bases at a specific position, this can be achieved by designing separate oligos, each containing a different base at the position of interest.

Twist can synthesize oligos from 20 nucleotides up to a maximum of 350 nucleotides.  

Currently, our maximum oligo length is 350 bp.

Currently, the minimum length is 20 nucleotides for an oligo in an oligo pool order.

The turnaround time for oligo pools is 2-4 business days*

*Twist Internal Data 2025. Time frame of 2-4 business days for Twist Oligo Pools up to 300 nt is based on internal data available as of 2025. This timeframe refers to the typical processing and handling time within our facilities before your order is handed over to the shipping carrier. Actual delivery times will vary depending on your location, the chosen shipping method, and the carrier's handling procedures.

Pricing depends on the complexity tier (Pool Size) and the oligo length tier (20-120nt, 121-150nt, 151-200nt, 201-250nt, 251-300nt) of the oligo pool.

Pricing for oligo pools can be found on our website under your account.

If you need to contact your Account Manager regarding pricing, their contact information is available in eCommerce under Account.

We deliver unamplified single stranded DNA (>0.2 fmole per oligo), pooled in a single tube as dried down product. Each oligo will match the sequence provided by customer exactly, with an estimated error rate of 1:3000 nt.

1. Unmodified oligos are stable. For long-term storage (up to one year), freeze DNA at -20° C. For even longer storage, freeze DNA at -80° C.

2. If the oligos are stored in solution, avoid repeated freeze-thaw cycles as this process can lead to physical degradation of the oligo.

3. If numerous experiments are planned, aliquot the sample and store at -20°C.

4. Careful handling is recommended to avoid possible contamination with nucleases or bacteria.

5. Download the Twist DNA resuspension protocol.

Twist Bioscience ships its DNA products dried down or resuspended in 2 mL microcentrifuge tubes, 96-well plates, or 384-well plates. Dried DNA is shipped at ambient temperature and resuspended DNA is shipped frozen.

Double-stranded DNA and single-stranded oligonucleotides are stable under most standard laboratory storage conditions. However, it is important to consider the following best practices to maintain the high quality of the DNA synthesized by Twist Bioscience.

• Twist recommends storing Dried DNA for the following durations based on storage condition.
  • Room Temperature for 3 months.
  • 4° C for 12 months.
  • -20° C for 24 months.
  • -80° C for long term storage.
• Resuspension Guidelines
  • For resuspension, briefly centrifuge the tube or plate before opening and resuspend in nuclease free Tris-EDTA (TE) buffer, pH 8.0 or 10 mM Tris-HCl, pH 8.0 to the desired concentration.
  • A concentration of at least 10 ng/μL is recommended for the stock dilution, but the optimal concentration will need to be determined based on your desired application.
  • Prepare aliquots of the stock dilution and separate working aliquots to limit chances of contamination and to reduce the number of freeze/thaw cycles. Use working aliquots as soon as possible after preparation and minimize exposure to high temperatures.
• Resuspended DNA
  • Resuspension is available for some products. Your DNA may arrive in one of the following buffers. We recommend briefly centrifuging the tube or plate before opening.
    • Elution buffer (2 mM Tris-Cl, pH 8.5)
    • TE buffer (10 mM Tris-Cl pH 8.0, 1 mM EDTA)
    • TE Buffer low EDTA (10 mM Tris-Cl pH 8.0, 0.1 mM EDTA)
    • Water       
      • We do not recommend long-term storage in water.
  • Twist recommends storing Resuspended DNA for the following durations based on storage condition.
    • Room Temperature for 3 months.
    • 4° C for 12 months.
    • -20° C for 24 months.
    • -80° C for long term storage.

Twist DNA products are dried down and shipped in either 96/384-well plates or 2 ml microcentrifuge tubes. Although double-stranded DNAs are stable under most standard storage conditions, we recommend the following to maintain high quality DNA:

• Upon receipt, briefly centrifuge tube or plate and resuspend the DNA in nuclease free Tris-EDTA (TE) buffer, pH 8.0 or 10 mM Tris-HCl, pH 8.0 to the desired concentration.
• We do not recommend resuspension in water.
• A concentration of at least 10 ng/ul is recommended for a stock solution, but optimal concentration will need to be determined based on your desired application.
• Prepare aliquots of the stock and separate working aliquots to limit the chances of contamination and reduce the number of freeze/thaw cycles.
• For long term storage, freeze DNA at -20° C for up to one year, or at -80° C for even longer storage.

Download the DNA resuspension protocol here.

Twist amplification guidelines for oligo pools can be found here.  Contact us at customersupport@twistbioscience.com if you have additional questions.

Twist's oligo pools are a user-designed collection of single stranded DNA oligonucleotides, ranging from 20 - 350 nt in length. The oligo pools are synthesized at high uniformity, with >90% of sequences present at signals within 2.5x of the mean, ensuring high uniformity and low dropout rate.

Additionally, industry-leading error rate of 1:3000 ensures the sequences that are ordered is what is delivered.

Each oligo is present at > 0.2 fmol per oligo

Twist's oligos have an average error rate of about 1:3000 or less.  For more information, click here.  

Certificates of Analysis (COA) are available for completed orders. You can find the COA for Oligo Pool products in your account by clicking here.