In the last 40 years, advances in protein engineering and evolution have prompted the application of biocatalysts in the synthesis of active pharmaceutical ingredients (API), as well as fine and bulk chemicals for the agrochemical, food, biofuel, and pharmaceutical industries. Computational chemistry methodologies in conjunction with smart variant library generation and high-throughput screening are fueling the development of a new generation of rationally designed biocatalysts with enhanced selectivity and specificity at a fraction of the time and cost of what was achievable a mere decade ago.

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Cell-free protein expression can accelerate discovery in synbio. Gene fragments are an optimal substrate for cell-free protein production