This Ancient Human DNA Target Enrichment Standard Hybridization protocol generates enriched DNA libraries for sequencing on Illumina next-generation sequencing (NGS) systems. This protocol details the steps for a 16-hour hybridization in a two-day target enrichment workflow and is optimized for use with the Twist Ancient Human DNA Panel.

In the era of precision medicine, next-generation sequencing (NGS) has become a vital tool for comprehensive genomic profiling (CGP) of solid tumors. However, with a broad spectrum of therapies already approved or in clinical trials, it is essential to develop rapid and scalable techniques capable of detecting an evolving number of therapeutically relevant genomic alterations and complex biomarkers.To meet these needs, OncoDNA, a world-leading theranostics company, developed OncoDEEP — an all-in-one CE-IVD approved assay supporting the delivery of personalized treatment strategies. Curated by oncology experts and powered by Twist hybrid capture technology, the OncoDEEP NGS panel delivers accurate profiling of single-nucleotide variants (SNVs), copy number variants (CNVs) and insertions and deletions (indels) in 638 clinically relevant genes.