In the post-genome era, large-scale, gene-centric target discovery studies using tools such as RNAi and CRISPR have proliferated, and produced many insights into disease biology. Challenges remain however, as these tools often struggle to interrogate the subtle biological processes that govern clinical response. Fortunately, new screening tools exist that can both find targets and uncover the mechanisms that modulate complex molecular interactions. One such tool is SITESEEKER®, developed by the scientists at PhoreMost.  In this webinar you will: Hear how SITESEEKER® exploits protein interference (PROTEINi) to deliver a substantially greater magnitude of complexity than existing screening tools. See how the path from target ID to drug development can be shortened using PROTEINi, through the discovery of druggable pockets in addition to targets. Discover how this technology can be applied in key disease areas, including in cancer biology, neurodegeneration and for hijacking targeted protein degradation. Learn why the length and fidelity of Twist Oligo Pools is enabling PhoreMost to develop the next generation of their platform.

Functional genetic screening using the CRISPR/Cas9 system is a powerful tool for interrogating gene function at scale, but access to and implementation of this technology is constrained by existing pre-defined CRISPR sgRNA libraries. Here, a single Twist Oligo Pool was used in combination with Custom Library Multiplexed Cloning (CLUE; crispr-clue.de), an easy-to-use bioinformatics and wet-lab workflow, to generate numerous high-quality, custom sgRNA libraries. The exceptional fidelity of Twist Oligo Pools preserves the uniform representation of sgRNAs in the resulting CLUE libraries without cross-library contamination, meeting the stringent requirements for success in CRISPR screening applications.

A new, best-in-class scoring algorithm for efficacious sgRNA library production